Animal Models for Study of Colitis-associated Cancer/Colorectal Cancer
Creative Animodel provides one of the most comprehensive service for developing animal models of Colitis-associated Cancer/Colorectal Cancer. Our experienced experts and scientists provide one-stop service from model creation to severity assessment and preclinical drug efficacy evaluation. We offer the strategy and experimental design that best fit your needs and herein to accelerate the speed to achieve your research goals.
Creative Animodel provides the AOM/DSS mice models of Colitis-associated Cancer/Colorectal Cancer. AOM.DSS model has been proved to dramatically shorten the time needed for induction of CRC and is widely used in colorectal cancer research.
Creative Animodel also provides various assessment service, which includes, but not limited to:
- • Assessment of body weight loss
- • Assessment of colon length
- • Assessment of tumor size and tumor quantity
- • H&E stained slides
- • Endoscopic imaging
- • Assessment of pro-inflammatory cytokines including IL-1β, IL-6, IFN-γ, TNF-α
- • Assessment of Ki67+ and TUNEL+ including fluorescence staining
Colorectal cancer（CRC）is one of the most common human malignancies. It is the third most commonly diagnosed cancer in males and second most in females in developed countries including United States. Further, it is also the second and third leading cause of cancer death in males and females. It has been found that CRC is firmly linked to chronic intestinal inflammation, given rise to the term “colitis associated cancer (CAC)”. Inflammatory bowel disease (IBD), which is a group of inflammatory conditions along the gastrointestinal tract, has been reported to be one of the best characterized examples of an association between intestinal inflammation and carcinogenesis. Among Ulcerative Colitis (UC) patients, the risk of colon cancer increases from 2% at 10 years after initial diagnosis to 8% at 20 years and further up to 18% at 30 years. In contrast, the lifetime risk of sporadic colorectal cancer in the United States is only 5%. Compare to 8% at 20 years and 18% at 30 years, inflammation associated cancer has become a problem that needs to be aware of and worth to investigate.
Many animal models of sporadic and inflammation-related colon carcinogenesis have been developed for research including chemically induced CRC model, genetically engineered mouse models, and xenoplants. Among all these mice models, the combination of azoxymethane (AOM) with one week of exposure to the inflammatory agent dextran sodium sulphate (DSS) has been proved to dramatically shorten the time needed for induction of CRC. Moreover, such method rapidly recapitulates the aberrant crypt foci-adenoma-carcinoma sequence that occurs in human CRC. AOM/DSS model of CRC has many advantages such as high reproducibility and low price. Because of these advantages, it has been used as a common model today for study of colon carcinogenesis.
Creative Animodel is dedicated to create the best animal models for your research. Our experienced experts fully understand the importance of Colitis-associated Cancer/Colorectal Cancer research and they would work with you to find and create the appropriate mice model for your studies, help to set up a detailed research plan, provide service to evaluate the severity of colon carcinogenesis and efficiency of potential treatments.