Animal Models of Myeloma
A series of animal models have been generated to access pathophysiology of myeloma and to evaluate anti-osteoclastic, anti-osteoblastic or anti-tumor therapies in myeloma. Currently, three types of models are available in Creative Animodel: BALB/c mice injected with pristane oil, immunodeficiency mice injected with malignant plasma cells and C57BL/KalwRij mouse injected with allogeneic malignant plasma cells. With a large collection of data in animal experiments, we are able to give you useful and practical advice and provide viable> models to explore therapy approaches and new pathways of human myeloma.
Pristane-induced plasmacytoma in BALB/c mice
Intraperitoneal injection of mineral oils, such as pristane, in BALB/c mice is an approach we use to generate a myeloma model which can induce accumulation and proliferation of plasma cells (PCs) in the peritoneal cavity. This model is adapted to study the gene expression profile in PCs during tumor progression and the oncogenic rearrangements of c-myc, as well as to investigate the role of IL-6.
The 5TMM model in the C57BL/KalwRij mouse
Aging mice of the inbred strain C57BL/KalwRij have been identified as a suitable model to develop monoclonal B-cell proliferative disorders. The cell lines of myeloma are maintained in vivo, then are propagated by intravenous injection into young syngeneic mice. This model can be used in the studies in the following, but is not limited to:
● BM homing
● Osteolytic bone disease
● DNA vaccination
● Cytogenetic studies
Immunodeficiency mice: the SCID model
● SCID xenograft model: We inject human PCs in severe combined immunodeficiency mice to generate a xenograft model. Intravenous injection of some myeloma lines such as KPMM2 which have been well characterized in SCID mice is a commonly used approach to induce a major development within the hematopoietic bone marrow and lymph nodes.
● SCID-hu mice: This is a human myeloma model in a SCID mouse which can be used as a host for bone graft of human foetus, normal adult and myeloma patients. To create this model, we directly inject myeloma cells into the muscles surrounding the bone marrow grafts to represent morphological and immunologic features of human myeloma for preclinical drug studies.
Both single-transgenic mouse and double-transgenic can be developed as myeloma models by culturing cells in vitro. We can customize transgenic mice according to different biological and clinical features of models.
Thanks for many years’ experience in animal experiments, Creative Animodel are able to develop novel models and more effective treatment strategies to unravel the pathobiology of myeloma. We can also provide service of animal selection by evaluating the advantages and limitations of each model. To learn how we can help with individualized studies of human myeloma by supplying animal models, please feel free to contact us.