Collagen Antibody-induced Arthritis (CAIA) Model
As one of the best vendor of animal models in the world, Creative Animodel offers the most comprehensive service for developing animal models of Rheumatoid Arthritis. We have talented experts and scientists who have years of experience in inflammation model creation, model selection, and preclinical experiments. They would assist you on every step of your research and accelerate the speed to achieve your goals.
Mouse models have been developed and widely used for rheumatoid arthritis (RA) research to test new candidate therapeutic agents and investigate the pathogenic mechanisms of RA. To induce the chronic inflammation, several methods are used and different type of models are introduced including Collagen induced arthritis (CIA) model, Collagen antibody-induced arthritis (CAIA) model, and Adjuvant induced arthritis (AIA) model.
Among the different models, CAIA is a simple and synchronized alternative to the CIA model. The arthritis of this model is stimulated by the administration of a cocktail of monoclonal antibodies that are directed to conserved auto-antigenic epitopes in collagen type II, followed by endotoxin. The advantages of CAIA model compared to the CIA model is that CAIA model has rapid disease onset (24 to 48 hours), high uptake rate, synchronicity and lack of influence of Freund’s adjuvant, which was used for CIA model and affects the immune system. Therefore, this model is perfect for investigating the pathological role of individual gene products, pro-inflammatory and anti-inflammatory cytokines, screening and evaluating anti-inflammatory agents (Khachigian, 2006).
Figure 1. Collagen antibody-induced arthritis in mice. (a), A cocktail of arthritogenic mAb (M2139+CIIC1), isotype control (L243+G11) and PBS control. (b) after antibody transfer in B10.RIII mice (left, normal paw; right, arthritis paw), and (c) after antibody transfer and lipopolysaccharide injection in BALB/c mice (left, arthritis paw; right, normal paw). (Picture and legends from Dr. Nandakumar’s publication (Nandakumar and Holmdahl, 2006))
To induce the arthritis, a mixture of type II collagen specific antibodies (2.4-3.2 mg per animal) together with LPS (25 µg/mouse) will be made. On day 0, the 2.4 to 3.2 mg cocktail will be intra-peritoneal (i.p) administered to mice. Then, between day 3 and 7, LPS will be i.p administered to increase and synchronize the inflammation. Further, arthritis score and other symptoms will be observed and reported on daily basis. The induced arthritis shows the same clinical, histological and immunological features with the human rheumatoid arthritis including increase of pro-inflammatory cytokines such as TNF-α, IL-6, erosion of bones, and pannus formation.
Creative Animodel also provides various preclinical services which includes, but not limited to:
- • Clinical Score of arthritis assessment
- • Body weight measurement
- • Immunohistochemically evaluation of the arthritis pathology in joints
- • Evaluation of ankle and paw joint swelling
- • Assessment of major immune factors such as IL-1, TNF-α
- • Flow cytometric analysis of the inflammatory cells
- • PK blood collections
Creative Animodel is dedicated to create the best and most appropriate animal models of rheumatoid arthritis for our customers. We offer CIA, CAIA and AIA arthritis models and regular preclinical service. Moreover, we also provide customized model creation and clinical assessment service to meet all your specific needs.
Khachigian, L.M. (2006). Collagen antibody-induced arthritis. Nat Protoc 1, 2512-2516.
Nandakumar, K.S., and Holmdahl, R. (2006). Antibody-induced arthritis: disease mechanisms and genes involved at the effector phase of arthritis. Arthritis Res Ther 8, 223.