Food Intake Studies of Obesity
Creative Animodel provides comprehensive services for investigating the mechanism of obesity and novel therapeutics to treat obesity. One of the most widely used methods is feeding study includes acute feeding study, sub-chronic and chronic feeding study. Our scientific team has extensive experience in anti-obesity drug development and obesity mechanism investigation. We will assist you on each step of your feeding study for obesity.
Acute feeding study
Acute feeding study is the simplest and most commonly used measurement for screening the anti-obesity potential of a novel drug and for investigation of the mechanism of obesity. Furthermore, acute feeding studies are often used as the initial screen for centrally-acting drugs.
Key features of acute feeding studies:
- • Available in rat or mice (singly-housed)
- • Male lean or obese animals
- • Drug administration route could be various, such as po, sc, ip
- • Mice acclimatised to palatable wet mash (4 h/day). Drugs given 60 min before mash. Food intake normally measured over 1, 2 and 4 h.
- • Rats maintained under reverse-phase lighting with free access to normal, powdered rat chow. Drugs given at onset of dark period. Food intake normally measured for up to 24 h.
- • Compounds can be given repeatedly for up to 7 days if a delayed onset of action is suspected
- • Blood sampling for pharmacokinetic analysis if required
Behavioural specificity of the reduction in gross food intake can be investigated by:
- • Satiety profiling (mice or rat)
- • Pica (kaolin intake)
- • Conditioned taste aversion
Sub-chronic and chronic feeding study
To fully evaluate the effects of novel anti-obesity drugs and mechanism on body weight and food and water intake, sub-chronic or chronic feeding studies are commonly utilized. Feeding studies can be performed in normal rat/mouse and obese rat/mouse.
Food intake parameters normally include:
- • Daily body weight and food and water intake
- • Change in body weight per week
- • Average daily food and water intakes per week
- • Overall and cumulative food and water intake on each day of treatment
Sub-chronic and chronic feeding studies are customized and can include the following test:
- • Glucose challenge tests
- • Blood plasma level measurement
- • Pharmacokinetic analysis
- • Measurement of blood pressure
- • Urinary glucose excretion and faecal fat content
- • Pancreatic insulin levels measurement
- • Liver total cholesterol and triglyceride levels measurement
- • Body composition analysis
- • Ex vivo binding to confirm brain penetration/site of action
- • Necropsy and gross post mortem analysis
Creative Animodel offers our clients customized feeding studies to meet your specific requirements. Our service also includes advice on experimental design, statistical analysis by a qualified statistician, fully audited data pack and written reports to regulatory standards.