Cancer is one of global leading causes of death diseases and draws many researchers’ attentions for decades. Most anti-tumor and cancer therapeutic studies are carried out in rodent models due to rapid reproduction period, small size and low cost. However, species difference between rodent and human increase risk of failure during clinical trials. Non-human primates, in genetic and physiological aspects, are more advanced in preclinical efficacy and toxicity tests. Creative Animodel has expert personnel and enriched NHP resources for developing carcinogen-induced NHP tumor models.
Chemicals Induced Tumor Model
Compared with xenograft or genetically modified tumor model, chemical carcinogens induced tumor model can better display full spectrum of cancer pathogenesis, especially early stage, and has advantage of easy administration (IV, IP or orally). Creative Animodel provide chemical induced NHP cancer model and part of chemical carcinogens list as follow:
||2-Amino-1methyl-6-phenylimidazo [4,5-b] pyridine
2-Amino-3, 8-methylimidazo[4,5-f] quinoxaline
|Breast, Prostate tumor carcinogen
Liver tumor initiation
Liver tumor initiation
|Polycyclic aromatic hydrocarbon
|Liver tumor promoter
Breast tumor initiation
|Liver tumor initiation
||Liver tumor initiation
Please contact us for more chemicals induced cancer models.
Infection-associated Cancer model
There are about 17.8% of human cancer are caused by infectious agents such as bacteria, herpesvirus, hepatitis B and C virus. Creative Animodel provides effective model on studies of infection-associated tumor model. We have experience in developing lymphomas by using herpesvirus saimiri (HVS) and Epstein-Barr virus infected tamarin, and we also are capable of other infection-associated cancer models.
Example of Virus-associated Cancer model in NHP
1) Primate: Marmoset
2) Infectious agent: Kaposi's Sarcoma associated herpesvirus infection (human herpesvirus 8)
3) Viral Chanllege: Vero cell carrying rKSHV.219 was used to stimulate viral production. Virus-carrying cells were cultivated in 75nM of TSA solution for 24h and switched to DMEM media for another 48h. Virus was harvested by centrifuging at 2000rpm for 10min and filtered through 0.45µm filter membrane, supernatant was rotated for addition 3hr at 18000rpm. Collected viral inoculum was slowly delivered to marmoset by dripping viral inoculum into tonsils and nostril mucosa. Animal was anaesthetized during inoculation and swallow reflex was maintained under light treatment.
4) Viral detection, histopathological and immunological analyses.
5) Result: Orally infected marmoset displayed KS-lesion and spindle cell sarcoma showed positive KSHV DNA and protein