CRISPR/Cas9 System for Mutagenesis and Genome Editing
The latest tool in genome editing-CRISPR/Cas9- allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. It is analogous to the TALEN DNA nuclease system and can be used to induce mutations at targeted regions in a model organism's genome.
It is clear that the CRISPR/Cas system will be widely developed and used as a targeted mutation system in human cells and in mice/rat, due to its superior efficiencies and advantages over similar targeted cleavage systems.
Cas9 is targeted to DNA by a guide RNA that forms base pairs to itself and to the DNA target. Cleavage occurs on both strands (red X) 3 bp upstream of the PAM
Gene editing mice and rats
| Mice | Turnaround Time | Guarantee |
| Knockout | 5 - 7 months | At least 4 heterozygous mice |
| Conditional Knockout | 5 - 7 months | At least 2 heterozygous mice |
| Site-Specific Knockin* (<2.5 Kb) | 5 - 7 months | At least 2 heterozygous mice |
| Gene Mutation | 5 - 7 months | At least 2 heterozygous mice |
| Transgenic (Random Insertion) | 5 - 7 months | At least 3 transgenic mice |
| Rat | Turnaround Time | Guarantee |
| Knockout | 6 - 8months | At least 4 heterozygous rats |
| Site-Specific Knockin* (<2.5 Kb) | 6 - 8 months | At least 2 heterozygous rats |
| Gene Mutation | 6 - 8 months | At least 2 heterozygous rats |
| Transgenic (Random Insertion) | 6 - 8 months | At least 2 transgenic rats |
Gene editing cell lines
We can offer the following services
• Knockout cell lines
• Conditional Knockout cell lines
• Site-specific knock-in cell lines
• Gene Mutation cell lines
• Double gene knockout cell lines
• Gene tagging
Cell lines that we have been worked with
• HEK293 cells; CHO cells; CHO D44 cells
• HeLa cells; other tumor cells
• Mouse ES cells
If you are interested in other cells, please contact us to discuss your project.
Turnaround time
The turnaround time and efficiency varies depending on different factors, such as cell type, transfection efficiency and CRISPR/Cas9 activity.
Creative Animodel aims at making CRISPR/Cas9 technology accessible for everybody and is always looking for new ways to meet the needs of our valued customers. For more information, please contact us!
Our customer service representatives are available 24 hours a day, from Monday to Sunday.
