CRISPR/Cas9 System for Mutagenesis and Genome Editing

The latest tool in genome editing-CRISPR/Cas9- allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. It is analogous to the TALEN DNA nuclease system and can be used to induce mutations at targeted regions in a model organism's genome.

It is clear that the CRISPR/Cas system will be widely developed and used as a targeted mutation system in human cells and in mice/rat, due to its superior efficiencies and advantages over similar targeted cleavage systems.

Cas9 is targeted to DNA by a guide RNA that forms base pairs to itself and to the DNA target. Cleavage occurs on both strands (red X) 3 bp upstream of the PAM

Gene editing mice and rats

Mice	Turnaround Time	Guarantee
Knockout	5 - 7 months	At least 4 heterozygous mice
Conditional Knockout	5 - 7 months	At least 2 heterozygous mice
Site-Specific Knockin* (<2.5 Kb)	5 - 7 months	At least 2 heterozygous mice
Gene Mutation	5 - 7 months	At least 2 heterozygous mice
Transgenic (Random Insertion)	5 - 7 months	At least 3 transgenic mice

Rat	Turnaround Time	Guarantee
Knockout	6 - 8months	At least 4 heterozygous rats
Site-Specific Knockin* (<2.5 Kb)	6 - 8 months	At least 2 heterozygous rats
Gene Mutation	6 - 8 months	At least 2 heterozygous rats
Transgenic (Random Insertion)	6 - 8 months	At least 2 transgenic rats

Gene editing cell lines

We can offer the following services

•  Knockout cell lines
•  Conditional Knockout cell lines
•  Site-specific knock-in cell lines
•  Gene Mutation cell lines
•  Double gene knockout cell lines
•  Gene tagging

Cell lines that we have been worked with

•  HEK293 cells; CHO cells; CHO D44 cells
•  HeLa cells; other tumor cells
•  Mouse ES cells
If you are interested in other cells, please contact us to discuss your project.

Turnaround time

The turnaround time and efficiency varies depending on different factors, such as cell type, transfection efficiency and CRISPR/Cas9 activity.

Creative Animodel aims at making CRISPR/Cas9 technology accessible for everybody and is always looking for new ways to meet the needs of our valued customers. For more information, please contact us!

Our customer service representatives are available 24 hours a day, from Monday to Sunday.