Cas9 is targeted to DNA by a guide RNA that forms base pairs to itself and to the DNA target. Cleavage occurs on both strands (red X) 3 bp upstream of the PAM
Gene editing mice and rats
|Knockout||5 - 7 months||At least 4 heterozygous mice|
|Conditional Knockout||5 - 7 months||At least 2 heterozygous mice|
|Site-Specific Knockin* (<2.5 Kb)||5 - 7 months||At least 2 heterozygous mice|
|Gene Mutation||5 - 7 months||At least 2 heterozygous mice|
|Transgenic (Random Insertion)||5 - 7 months||At least 3 transgenic mice|
|Knockout||6 - 8months||At least 4 heterozygous rats|
|Site-Specific Knockin* (<2.5 Kb)||6 - 8 months||At least 2 heterozygous rats|
|Gene Mutation||6 - 8 months||At least 2 heterozygous rats|
|Transgenic (Random Insertion)||6 - 8 months||At least 2 transgenic rats|
Gene editing cell lines
We can offer the following services
• Knockout cell lines
• Conditional Knockout cell lines
• Site-specific knock-in cell lines
• Gene Mutation cell lines
• Double gene knockout cell lines
• Gene tagging
Cell lines that we have been worked with
• HEK293 cells; CHO cells; CHO D44 cells
• HeLa cells; other tumor cells
• Mouse ES cells
If you are interested in other cells, please contact us to discuss your project.
The turnaround time and efficiency varies depending on different factors, such as cell type, transfection efficiency and CRISPR/Cas9 activity.
Creative Animodel aims at making CRISPR/Cas9 technology accessible for everybody and is always looking for new ways to meet the needs of our valued customers. For more information, please contact us!
Our customer service representatives are available 24 hours a day, from Monday to Sunday.