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Circulating Tumor Cells-Derived Xenograft Models

Circulating tumor cells (CTCs) have been used to generate PDX models of breast cancer, colon adenocacinoma and prostate cancer so as to get access to a prediction and dynamic monitoring of tumor progression and metastasis. Creative Animodel provides customers model construction services for PDX engrafted with CTCs whereby combining CTCs enrichment system, which achieved by tumor specific biomarker-coupled ferrofluid nano-particles and cell characterization system such as FISH assay, with PDX engraftment system.

CTCs are generally considered to be cells leaked out of a primary tumor and are carried around the body in the circulation and then give rise to tumor metastasis (one of the most dangerous aspects of cancer), triggering a mechanism that is responsible for the vast majority of cancer-related deaths. Early identification and characterization of CTCs is essential to perceive the sign of metastasis and to execute an early intervention.  Compared to tissue biopsies, CTCs are considered to be a liquid biopsy which providing dynamic and live information about tumor progression with little harm to the patients. To date, technologies with the requisite sensitivity and reproducibility to detect, enumerate and enrich CTCs in patients have been explored.

Correspondingly, PDX models from CTCs have many advantages over conventional PDX models including less harm to patients by virtue of the no-surgery sample collection approach and realizing dynamic disease monitoring by generating experimental models at different stages.

Circulating Tumor Cells-Derived Xenograft Models

Creative Animodel has established mature methodologies to develop CTCs-derived PDX models of prostate cancer, colon adenocarcinoma as well as breast cancer. We are making progressive elaboration by improving techniques of CTCs enrichment, which include the following categories, but are not limited to:

  • • Positive and negative selection by CTCs surface antigen staining
  • • Screening and filtration by CTCs size
  • • Detection by CTCs molecular characterization

Among the referred methods above, positive and negative selection is particularly well-established in Creative Animodel, which can be gradually achieved through density gradient centrifugation, red blood cell lysis, negative selection by flow cytometry depletion of CD45 positive mononuclear cells and finally enrichment of EpCAM positive CTCs from peripheral blood samples. The CTCs collected are then either for molecular characterization (by immunohistochemistry and molecular genomics analyses such as fluorescence in situ hybridization PCR, and single nucleotidepolymorphism analysis), or for further engraftment into highly immunocompromised mice to generate xenografts for functional studies.

Creative Animodel has established sensitive and specific CTCs analyzing relevant technique platform to help cancer researchers accurately characterize CTCs from a number of cancers (including leukemia, breast cancer, lung cancer, prostate cancer and colorectal carcinoma) by utilizing a broad range of cancer specific biomarkers, which enabling either single detection of distinct CTCs or simultaneous detection of different CTCs types in a multiplexed fashion (likely to elaborate the main mechanism of tumor heterogeneity). Creative Animodel also renders customized services like validated testing aiming at the development for other interested tumor specific biomarkers.

* For research use only. Not intended for any clinical use.

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