Creative Animodel is the leading services provider for obesity mechanism study and anti-obesity drug development. We have extensive experience on obesity study based on Dietary-induced obese (DIO) mice and rats and high fat diet mice/rats. We will assist you on each step of your study of obesity.
Dietary-induced obese (DIO) mice and rats are well-established animal models of obesity. The primary cause of obesity is excessive caloric intake with too little exercise. Dietary-induced obesity (DIO) in mice and rats usually involves a high-fat, high-carbohydrate diet and is translatable to the human population. Furthermore, animals with all genetic background could be the model for DIO animal.
Dietary-Induced Obesity (DIO) mouse model:
Background strain: C57BL/6 mice or other strain, Gender: male, Diet: 60% fat, Time on diet: 3-4 months
Dietary-Induced Obesity (DIO) rat model:
Background Strain: Sprague Dawley (SD) rat or other strain, Gender/Weight: male 250/300g, Diet: High fat - high carbohydrate, Time on diet: ~8 weeks (depending on required severity)
Key features of our dietary-induced obese rat and mouse models:
✓ Weight-stable (allowing weight-loss as opposed to reduced weight gain to be measured)
✓ Marked visceral adiposity (30-35% fat)
✓ To mimic gradual body weight gain, and especially visceral fat hypertrophy, as observed in western populations
✓ To select the best drug candidate in a reproducible model, suitable for screening
✓ High leptin levels
✓ Moderate insulin resistance (rather than overt diabetes)
✓ Mild lipid abnormalities
✓ Polygenic basis
High fat fed overweight rat/mice model
Background strain: Sprague-Dawley rats or C57BL/6 mice, Gender: male, Diet: high fat diet (60% fat), Time on diet: two weeks, Animals in active growth phase
Key features of high fat fed "overweight" rat/mice model:
✓ An excellent polygenic screen for investigating the efficacy and tolerability of drugs before chronic feeding studies in dietary-induced obese rats/mice.
✓ It does not include a lengthy induction of obesity period therefore animals are readily available.
✓ Pharmacological model relevant to the main classes of approved anti-diabetic compounds
Acute, sub-chronic and chronic feeding studies can be performed in DIO mice/rats and high fat diet mice/rats, and drugs can be given by a variety of routes. Body weights and daily food and water intakes are recorded routinely. Studies in dietary-induced obese rats and mice can include a number of other optional extras to investigate the effects of drugs on obesity-related comorbidities or to investigate the mechanisms underlying the weight-loss, including but not limited to:
✓ Glucose tolerance tests before, during and at the end of treatment
✓ Plasma analysis of a variety of metabolic parameters
✓ Blood pressure measurements
✓ Fat pad weights
✓ Body composition analysis
✓ Faecal fat analysis
✓ Urinary glucose excretion
✓ Blood and tissue-sampling for pharmacokinetic analysis
✓ Necropsy and post mortem analysis
✓ Histopathology and Immunohistochemistry
With Creative Animodel, all studies in dietary-induced obese mice/rats and high fat diet mice/rats can be customized to meet your specific requirements and include advice on experimental design, statistical analysis by a qualified statistician, fully audited data pack and written reports to regulatory standards if required.