Silencing lentiviral vectors and adenoviral vectorsTransgenic mice can be generated rapidly by transduction of early embryos with lentiviral silencing vectors and adenoviral vectors, resulting in mice with down regulated target genes. These methodologies offer the possibility of large-scale generation of knockdown transgenic mice for functional genomic studies and enable the production of transgenic mice in 7 weeks.
Cre/loxP system as an activate RNAi in a time- and tissue-dependent mannerSingle-copy RNAi constructs are placed into the Rosa26 locus of ES cells by recombinase-mediated cassette exchange and transmitted through the germline of chimaeric mice. The site-specific insertion of single-copy shRNA vectors allows the expedite and reproducible production of knockdown mice and provides an easy and fast approach to assess gene function in vivo.
Creative Animodel's proprietary RNAi technology enables you to induce and reverse ubiquitous gene knockdown in mice at selected points in time! Contact us!