Chimeric antigen receptor T (CAR-T) cell is one of the current leading immuno-therapy in cancer treatments. Increasing number of CAR-T involved clinical trials against CD19+ B cell lymphoblast leukemia have been conducted across multiple institutions and the outcomes are encouraging. With more CAR-T involved clinical trials in planning, preclinical pharmacological study of CAR-T cells will provide valuable information for the subsequent clinical studies and future therapies.
Creative Animodel offers comprehensive pharmacological study of CAR-T cell therapy using human tumor xenograft mouse or rat models. We provide customized services to help you design the experiments that best suite your study. With our experiences in animal model construction and preclinical studies, we will provide you with cost-effective services and quality data.
CAR-T preclinical studies we offer:
Pharmacokinetic study of CAR-T cells monitor the level and duration of CAR-T cells circulating in the blood stream. The longer CAR-T cells stays in blood circulation, the more effective each dose of CAR-T cells would be. In some cases, CAR-T cells were cleared out of the blood stream even before they could take effects, which leads to a failed treatment.
Aspects of CAR-T cell pharmacokinetic study
✓ Serum persistence of CAR-T cells in the blood stream
✓ Engraftment in bone marrow
✓ Biodistribution of CAR-T cells in different organs
CAR-T cells efficacy study analyzes the rate of tumor remission after the administration of CAR-T cell therapy.
Aspects of CAR-T cell efficacy study
✓ Time course tumor cell counts monitoring
✓ Time course solid tumor size monitoring
✓ Tumor size monitoring by bioluminescent imaging
✓ Animal necropsy and histological analysis of tissues
✓ Survival analysis of tested animals
Toxicity analysis of CAR-T therapy focuses on the aspects of CAR-T induced side effects, which including cytokine storm syndrome, Tumor cell necrosis and inflammation, and normal cell aplasia.
Aspect of CAR-T toxicity study
✓ Cytokine release analysis
✓ Tumor lytic analysis
✓ Normal cell aplasia analysis
Technology we used:
o Flow cytometry analysis
o Bioluminescent imaging
o ELISA/ antibody array for cytokine analysis
o Kaplan Mire survival analysis
Our state-of-art technology and experienced service team will assist you in assay selection and protocol development that best suit your study.