ADME Services

In vivo
Creative Animodel offers a suite of in vivo ADME services and features an in vivo team with over 10 years of experience. We are equipped to conduct in-life studies in rat (intact or bile/jugular vein cannulated), mouse and dog. Creative Animodel provides comprehensive radiolabel ADME study support across non-clinical species.

Services include:
• Pharmacokinetic Analysis of Blood (Plasma)
• Rodent Studies Using Radiolabelled Materials
• Metabolite Isolation and Characterization
• Bioavailability Evaluations
• Bioequivalence Studies
• Formulation Screening and Optimization
• Others...

In vitro
With extensive knowledge of in vitro ADME and pharmacokinetics gained through working within drug discovery environments at both pharmaceutical and biotech companies, our ADME experts offer a consultative approach ensuring that the right experiments are performed at the right time. Creative Animodel offers a range of over 200 assays for rapid evaluation of the pharmaceutical properties and safety of drug discovery compounds:

Physicochemical Studies

Creative Animodel offers physicochemical property determination services for small molecules, oligonucleotides, peptides, and industrial and consumer chemicals.
Solubility--- The turbidimetric solubility of your test agent in various buffers, cell culture media, or biological fluids will be determined. Thermodynamic solubility will be determined by HPLC or LC-MS/MS analysis.
Chemical Stability--- Test agent is incubated in artificial gastric or intestinal fluids to simulate the fed or fasted states. Moreover, the stability of a test agent in various buffers and across a pH range is measured.
PAMPA Permeability--- Measures the permeability of a test agent across an artificial membrane.

Absorption/Distribution Assays

Absorption can occur either through passive transcellular diffusion or through active carrier transport mechanisms. Distribution processes are also very important in influencing tissue distribution and can be major mechanisms of excretion.

Creative Animodel offers an extensive portfolio of assay-based products and services including:

• Pampa (Gi/Bbb)
• Caco-2 (Unidirectional/Bidirectional)
• Efflux Transpo
• Rters-Pgp/Bcrp
• Protein Binding (Ultrafiltration and Equilibrium Dialysis)
• Tissue Permeability Studies-Franz Diffusion Assay
• Blood/Plasma Partitioning Ratio

Physicochemical Studies

There is a significant advantage to obtain metabolism data as early as possible in the drug discovery process. Understanding how a drug compounds' enzymatic metabolic pathways and metabolic rates influence its clearance, metabolite concentration, and potential drug-drug interactions is a critical component to developing safe and effective drugs.
At Creative Animodel, a variety of assay matrices, including pooled liver microsomes, pooled liver S9 fraction, hepatocytes, human recombinant cytochrome P450s, UGTs, blood and plasma, are used for the metabolic assessment of hits, leads, and new pharmaceutical compounds.

Drug-drug interactions

Drug-drug interaction is a modification of the effect of a drug when administered with another drug. This can alter the way one or both of the drugs act in the body, or cause unexpected side effects. Early stage screening of compounds for potential drug-drug interactions using in vitro techniques becomes necessary in order to decrease late stage compound attrition. Creative Animodel has developed a set of in vitro tests which address:

• • Specific Chemical Inhibitors
• • CYP Inhibition
• • Drug Substance-Based CYP Inhibition
• • Fluorescence-Based CYP Inhibition
• • Cocktail Substance-Based CYP Inhibition
• • Time-Dependent CYP Inhibition
• • CYP Induction (Human Primary Hepatocytes and PXR Reporter Gene Assays)
• • Drug Substrate-Based DDI Potential Evaluation

Contact us to learn more and discuss appropriate applications.

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