Brain Tissue Binding
Creative Animodel offers a fast high throughput method to estimate brain tissue binding on drugs and predict their dispositions in brain. We can deliver consistent and high-quality data with flexibility to adapt protocols based on customers’ requirements.
Why Is Brain Tissue Binding Important?
Our Brain Tissue Binding Assay
For brain tissue binding, both standard and high throughput methods using brain homogenates have been developed to determine the fraction unbound (fu) of drugs in brain tissues at Creative Animodel. Good predictability and ease of use make brain homogenate binding one of the most widely used methods for determining fraction unbound in brain tissues. Our assays are accomplished by following three steps: preparation of brain homogenates, equilibrium dialysis for brain binding study and calculation of fraction unbound.
Calculation of Fraction Unbound. fu is calculated using equipments. Because unbound fractions determined from diluted brain homogenates are higher than those from undiluted brain tissue, corrections are made on the basis of the dilution factor (D). Masses in receiver and donor are determined from the area counts in these samples obtained from LC-tandem mass spectrometry analysis corrected to account for sampling volumes. A dilution factor of 5 (D=5) is applied in the calculation that reflects the dilution of the brain tissue homogenates.
• High throughput and ease of handling
• Low cost and with less resources needed
• Behavior difference monitering between species
Creative Animodel has experienced experts in the field of preclinical drug evaluation. If you have any questions, please feel free to contact us.
1. Di L.; et al. Species independence in brain tissue binding using brain homogenates. Drug Metabolism & Disposition the Biological Fate of Chemicals. 2011, 39(7):1270.