Compound Distribution Assays
Creative Animodel provides a set of in vivo compound distribution assays in support of lead candidates selection. We can assist you to identify the metabolic pathways, potentially targeted organism and elimination mechanisms of your compounds at an early stage. Autoradiographic technique, such as autoradiography, quantitative whole-body autoradiography (QWBA) and microautoradiography, is available to provide high-resolution images of the spatial distribution and match tissue concentrations of drug-related radioactivity throughout the body of laboratory animals. Furthermore, using proprietary software, quantitative data can also be produced.
The extent of drug penetration into the tissues is characterized by the volume of distribution (Vd). Different types of Vd can be determined from blood concentration versus time data. This can be defined as ‘the volume of fluid that is required to dissolve the amount of drug in the body (A) to give the same concentration as that in plasma at that time’. In other words:
where C is the plasma concentration. But the actual processes determining the magnitude of the volume of distribution are tissue permeability, which is related to physicochemical properties of the drug and the cellular membranes of different tissues, and perhaps most importantly, the affinities of the drug to plasma and tissue proteins. Thus, the distribution of compound is a critical step in PK research.
Tissue Distribution Assay
The bloodstream carries drug molecules throughout the body. Distribution of drug into non-target tissues serves as a depot of the drug that affects PK volume of distribution and PK half-life. Tissue distribution assays can give a prediction of pharmacokinetics and bioactivity in human body.
Blood Partitioning Assay
Certain drugs have the potential ability to permeate red blood cells could lead an increase of blood to plasma ratios. Blood partitioning assay advances the knowledge of compounds’ pharmacokinetic behavior.
Mass Balance Study
The primary objectives of a mass balance study are generally to determine the ratio of drug to metabolites in circulation and investigate the primary route of excretion, which is essential in pharmaceutical development.
Creative Animodel has a team of scientists expertized in compounds distribution to support your research and project needs at the location of your choice. Our mission as your partner is to deliver high-quality image and reliable data accurately and efficiently to further optimize the drug development process. We look forward to working with you in the future.