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Compound Distribution Assays

Creative Animodel provides a set of in vivo compound distribution assays in support of lead candidates selection. We can assist you to identify the metabolic pathways, potentially targeted organism and elimination mechanisms of your compounds at an early stage. Autoradiographic technique, such as autoradiography, quantitative whole-body autoradiography (QWBA) and microautoradiography, is available to provide high-resolution images of the spatial distribution and match tissue concentrations of drug-related radioactivity throughout the body of laboratory animals. Furthermore, using proprietary software, quantitative data can also be produced.

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What Is Compound Distribution?
Upon entry of drug into the systemic circulation, it is transported to organs and tissues by the blood, which process is called distribution. The majority of drugs have to be distributed to their site(s) of action. Only rare examples such as anticoagulants, heparin and the like, which have their effects in the bloodstream, do not. Drugs are carried by the circulation, often bound to plasma proteins from where they equilibrate with their sites of action or other storage sites. The extent and rate at which a drug is distributed are dependent on its physiochemical characteristics. Small lipophilic drugs that readily penetrate membranes are generally widely distributed, whereas polar, ionized ones and macromolecules are often contained in particular anatomical volumes.

The extent of drug penetration into the tissues is characterized by the volume of distribution (Vd). Different types of Vd can be determined from blood concentration versus time data. This can be defined as ‘the volume of fluid that is required to dissolve the amount of drug in the body (A) to give the same concentration as that in plasma at that time’. In other words:

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where C is the plasma concentration. But the actual processes determining the magnitude of the volume of distribution are tissue permeability, which is related to physicochemical properties of the drug and the cellular membranes of different tissues, and perhaps most importantly, the affinities of the drug to plasma and tissue proteins. Thus, the distribution of compound is a critical step in PK research.

Our Services

Tissue Distribution Assay
The bloodstream carries drug molecules throughout the body. Distribution of drug into non-target tissues serves as a depot of the drug that affects PK volume of distribution and PK half-life. Tissue distribution assays can give a prediction of pharmacokinetics and bioactivity in human body.

Blood Partitioning Assay
Certain drugs have the potential ability to permeate red blood cells could lead an increase of blood to plasma ratios. Blood partitioning assay advances the knowledge of compounds’ pharmacokinetic behavior.

Mass Balance Study
The primary objectives of a mass balance study are generally to determine the ratio of drug to metabolites in circulation and investigate the primary route of excretion, which is essential in pharmaceutical development.

Creative Animodel has a team of scientists expertized in compounds distribution to support your research and project needs at the location of your choice. Our mission as your partner is to deliver high-quality image and reliable data accurately and efficiently to further optimize the drug development process. We look forward to working with you in the future.

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45-1 Ramsey Road, Shirley, NY 11967, USA 1-631-614-7828 1-631-372-1052 Europe 44-207-097-1828

Creative Animodel

Creative Animodel is a world's leading commercial provider of custom animal model services. We offer top-quality services at unbeatable prices. Our customer service representatives are available 24 hours a day, from Monday to Sunday.

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