Isotopic Labeling Service
Creative Animodel is a leading service provider that focuses on developing isotopic labeling for the research of our clients. We provide stable isotopic labeling service to help you analyze drug metabolism process.
What Is Isotopic Labeling?
Isotopic labeling is an analytic technique that is used to track the movement of isotopes through reaction networks. For isotopic labeling technology, a specific atom of the reagent moieties is replaced with labeled isotopic variant, then, the position and relative abundance of labeled isotopic atoms are determined by experiment. Subsequent analysis of the measured quantities elucidates the characteristics of the reaction network. The experiment flow of 13C-labeling technique is shown in figure 1, it provides a means to simply and directly assess the bio-behavior of unaltered carbon nanomaterials (including fullerene), which would largely benefit evaluation of the biosafety and environmental fate studies of carbon nanomaterials.
Figure 1. Schematic illustration of experimental design (Xue-Ling C. et al, 2014): We labeled fullerene C60 with 13C isotopes on the skeleton and quantified the 13C-enriched C60 in mice. (a) 13C-enriched C60 was prepared by the arc discharge method and dispersed in tween 80 aqueous solution for the animal experiments. (b) After i.v. injection, blood and tissue samples were collected to analyze the 13C-enriched C60 content. (c) The isotope ratio mass spectrometry (IRMS) results indicated that 13C-enriched C60 cleared from blood quickly and was trapped in the organs of the reticuloendothelial system (RES).
An injection of a radioisotopically labeled drug can be used to determine blood, plasma, or urine levels and their temporal profile by liquid scintillation or gamma counting and measurement of activities in serially derived samples. Liquid scintillation counters can be used to measure total sample radioactivity. In addition, radioactivity of individual components can be determined after chromatographic separation by using an online radiometric chromatographic detector or by collecting chromatographic fractions and using a liquid scintillation detector. Scientists can trace the detailed process of chemical reactions by isotopic labeling, which has been widely used in the clinical research of medical field.
Types of Isotopic Labeling
▪ Uniform labeling with the stable isotopes 13C, 15N, and 2H
Target-based NMR screening relies on the detection of perturbations of target resonances on ligand binding. The most well-known technique is the SAR by NMR method. Ligands are identified by monitoring alterations of target signals in a 2D 1H–15N correlation spectrum. This method requires large quantities of isotopically (13N)-labeled protein (50–200 mg, with protein solubility between 0.1 and 1 mM). This method has the benefit (provided that sequence-specific resonance assignments have been obtained) of high reliability, being able to identify the binding site on the target, readily distinguishing specific from nonspecific interactions, and assessing whether any significant conformational changes occur upon binding. The incorporation of 13C into the target protein may also be used for the detection of binding of small molecules to targets.
▪ Radioisotopic labeling
We provide different kinds of sensitive radioactive isotopes and precise quantification of proteins.The chemical amount of our radioactive labeled compound is very small, it would not cause damage to the body physiological process.
Labeled Molecule Catalog
|001||Carbon||12C||Carbon precursors that contain only specific 12C-labeled sites are incorporated into the protein expression media||$|
|002||Carbon||13C||Carbon precursors that contain only specific 13C-labeled sites are incorporated into the protein expression media||$|
|003||Carbon||14C||Carbon precursors that contain only specific 14C-labeled sites are incorporated into the protein expression media||$|
|004||Nitrogen||14N||Tracing and distinguishing the source and fate of 14N species||$|
|005||Nitrogen||15N||Tracing and distinguishing the source and fate of 15N species||$|
|006||Oxygen||16O||16O Stable isotopes are mainly used for nuclear magnetic resonance, biomarkers and other scientific research||$|
▪ All the products and services here are available at low cost.
▪ Our measurement can accurately quantify and locate your object.
▪ Isotopic labeling service we offered can provide you with more objective results.
Creative Animodel has focused on developing isotopic labeling for many years. We provide different kinds of isotopes that can be used in the analysis of chemical reactions, our efficient and accurate results would help you continue study drug metabolism process. We promise that our professional experts will try their best to satisfy your needs.
1. Mark, I.B.; et al. "Modeling framework for isotopic labeling of heteronuclear moieties" Journal of Cheminformatics. 2017, 9:14.
2. Xue-Ling, C.; et al. "Quantification of carbon nanomaterials in vivo: direct stable isotope labeling on the skeleton of fullerene C60" Royal Society of Chemistry. 2014, 1, 64-70.