Label Free Methods
Creative Animodel is a leading biotechnology company, offering label-free methods for the clients to analyze protein expression and study the process in drug discovery. Based on our rich pharmaceutical experience and advanced LC-MS and LC-MS/MS technology, we can guarantee you the high-quality label-free services for effective peptide signals observation, and facilitating your project research.
What Is Label Free Methods?
Label-free techniques are methods for analyzing the relative amount of proteins in different biologic sources, by comparing the mass spectrometry number or mass spectral peak intensity. They can facilitate biomarker discovery involving a large number of samples, as the sample processing is straightforward. Both the LC-MS and LC-MS/MS methods are available here to combine liquid chromatography (LC) with mass spectrometry (MS) and for our clients to gain more sample information. Except for the differences in the data analysis process of this two experimental strategies, the other main distinct is on whether using tandem mass spectrometry to identify peptides and proteins. Label-free quantitative techniques are commonly used to identify peptides and proteins in biological samples and get high reputation by scientists in quantitative proteomics research and drug metabolism studies.
Fig 1. An overview of the label-free methods workflow.
Label Free Methods at Creative Animodel
The label-free LC-MS method demonstrates excellent proteome coverage and analytical reproducibility. We provide LC-MS technology to measure the various components and determine the detailed protein structure. Our excellent technology can be used to analyze your precursor ion, for example in an ion trap analyzer, a triple quadrupole and a time-of-flight mass spectrometry.
LC-MS/MS is also mentioned as tandem mass spectrometry, two-dimensional mass spectrometry and sequential mass spectrometry. It has become the most flexible technique for multiplexed protein quantification, and being regarded as the setup that can maximize proteome coverage. We offer high-sensitive LC-MS/MS technique for different product ions and precursors. Our LC-MS/MS service can help you to study the relationship between the ion and the parent ion, and then observe the structural information of the molecular ion to determine the composition of your sample.
• Accurately quantify your sample compounds
• Higher sensitivity and exceptional specificity
• Rapid turnaround period and stable label-free services
Creative Animodel has spent years of hardworking in the field of label-free techniques. We have an experienced in-house team and advanced technology instruments to provide high-quality services for our customers. Our technical supports are glad to help you solve problems from initial experimental design to final data processing. If you have any additional inquiries, please do not hesitate to contact us. Creative Animodel is the best partner of your scientific research.
1. Sandin, S.; et al. "Is label-free LC-MS/MS ready for biomarker discovery?" Proteomics - Clinical Applications. 2015, 9 (3-4): 289-294.