Ussing Chamber Assay
Creative Animodel provides Ussing chamber assay to predict compound absorption, permeability and excretion in intestinal tract. We have advanced technology platforms and experienced experts to guarantee the reliability of results.
Applications of Ussing Chamber
An Ussing chamber is an apparatus for measuring epithelial membrane properties. Danish zoologist and physiologist Hans Henriksen Ussing first introduced Ussing chambers to study the active transport of sodium in isolated frog skin. Since then, the apparatus has been improved into three main types: the classic chamber system, the self-contained chamber system, and the multi-channel chamber system.
Ussing chambers are used for three main purposes:
• To investigate drug absorption, transport and metabolism in an intact tissue.
• Serve as an electrophysiology method to investigate the role of ion channels in mucosal physiology and pharmacology.
• To investigate drug-mediated changes within the tissue (e.g. intracellular signaling) or the release of mediators from the tissue (e.g. the release of GLP-1 from the gut in response to glucose).
Mechanism of Ussing Chamber Assay
The technique is applied to opened, isolated intestinal sections mounted between two halves of a diffusion chamber. Stripping off the muscle layer is advantageous, as this removes the artificial permeability barrier, and the stripped tissues can also be oxygenated more efficiently. The compound of interest is introduced into the donor chamber and transport across the mucosa into the receiver chamber. The intestinal section, as a flat sheet, separates the buffers from the drug containing buffers so that the transport of molecules across the tissue and between the chambers can be measured. Gas flow of 95% O2 and 5% CO2 provides a continuous stirring of the buffer solution, reducing the unstirred layer in the two sides of the chambers and supplying oxygen. The accumulation of the test compound in the receiver chamber is measured as a function of time, and the rate of accumulation is normalized for the tissue surface area. The apparent permeability coefficient (P) is estimated using the following equation:
where V is the volume of the receiver chamber, A is the exposed tissue surface area, C is the initial concentration of the drug in the donor chamber, and dC/dT is the change in drug concentration in the receiver with time. Both mucosal-to-serosal and serosal-to-mucosal transport can be characterized by this method.
Creative Animodel is a leading biotech company. We provide a range of services for biopharmaceutical companies in the process of early drug evaluation and screening. Our staff and experts strictly observe the high quality standards to guarantee the reliability of final data. If you have any questions, please feel free to contact us. We are glad to establish cooperative relationship with you.
1. Tihanyi.; et al. Solubility, delivery and ADME problems of drugs and drug-candidates. Bentham Science Publishers, 2011.