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Whole Blood Binding Assay

Creative Animodel offers whole blood binding assay to help our clients to identify if differential binding to a specific component in the blood occurs, and interpret pharmacokinetic data. Our assays can also be used to investigate non-linear pharmacokinetics via saturation of blood binding.

Why Whole Blood Binding?

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Blood is the real fluid in which drugs are transported so it is significant to address the concept of “free concentration in blood”: clearly, this is the concentration of compound not bound to plasma protein or to blood cell. Circulating free drug is in equilibrium with plasma proteins and with blood cells in the vessels. The in vitro test in plasma reduces this 3-component equilibrium to a 2-component one. If there are non-linear processes, the equilibrium among free concentrations, bound concentrations in blood cells and bound concentrations in plasma may change in a way which would not be predictable from in vitro experiments in plasma only. So the results of in vitro test in plasma may be misleading.
It must be noted that all blood cells, not just erythrocytes, can bind drugs. Using whole blood to address both partitioning and protein binding processes can provide a more realistic picture of what happens in vivo.
Our experienced experts propose that clinical ex vivo protein-binding studies should be performed on highly bound compounds (a definition of highly bound is suggested as > 95%). And if the compound is highly bound to protein or blood cell is non-linear in the tested interval, the experiment would be more appropriately performed in whole blood than in plasma.

Our Whole Blood Binding Assay

Our whole blood binding assay is performed using an equilibrium dialysis method and delivers a fraction of compound unbound to blood (fu). Blood binding is determined by using a disposable RED plate preloaded with 48 disposable tube insert. Each insert is comprised of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane. Dialysis is performed against isotonic phosphate buffer saline (PBS) at pH 7.4. The test-compound presenting in each compartment is quantified by HPLC-MS/MS. In our assays, you can notice neither clotting, significant hemolysis or unacceptable pH variations, nor instability at 37℃.

Our Advantages
• High-throughput compound profiling
• Meet the customers’ special requirements
• Competitive price and fast turnaround time

Creative Animodel leverages our extensive expertise and integrated techniques to provide whole blood binding assays. With years of experience, our whole blood binding assays can provide results which are more relevant to the in vivo situation. If you have any additional requirements or questions, please do not hesitate to contact us.

Reference:
1. Pellegatti M.; et al. Plasma protein binding and blood-free concentrations: which studies are needed to develop a drug?. Expert Opinion on Drug Metabolism & Toxicology. 2011, 7(8):1009-20.

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45-1 Ramsey Road, Shirley, NY 11967, USA 1-631-207-8356 1-631-871-5806 Europe 44-207-097-1828

Creative Animodel

Creative Animodel is a world's leading commercial provider of custom animal model services. We offer top-quality services at unbeatable prices. Our customer service representatives are available 24 hours a day, from Monday to Sunday.

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