Whole Blood Binding Assay
Creative Animodel offers whole blood binding assay to help our clients to identify if differential binding to a specific component in the blood occurs, and interpret pharmacokinetic data. Our assays can also be used to investigate non-linear pharmacokinetics via saturation of blood binding.
Why Whole Blood Binding?
Our Whole Blood Binding Assay
Our whole blood binding assay is performed using an equilibrium dialysis method and delivers a fraction of compound unbound to blood (fu). Blood binding is determined by using a disposable RED plate preloaded with 48 disposable tube insert. Each insert is comprised of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane. Dialysis is performed against isotonic phosphate buffer saline (PBS) at pH 7.4. The test-compound presenting in each compartment is quantified by HPLC-MS/MS. In our assays, you can notice neither clotting, significant hemolysis or unacceptable pH variations, nor instability at 37℃.
• High-throughput compound profiling
• Meet the customers’ special requirements
• Competitive price and fast turnaround time
Creative Animodel leverages our extensive expertise and integrated techniques to provide whole blood binding assays. With years of experience, our whole blood binding assays can provide results which are more relevant to the in vivo situation. If you have any additional requirements or questions, please do not hesitate to contact us.
1. Pellegatti M.; et al. Plasma protein binding and blood-free concentrations: which studies are needed to develop a drug?. Expert Opinion on Drug Metabolism & Toxicology. 2011, 7(8):1009-20.