In Vitro Hypoxia Studies
Creative Animodel is a leading biological company dedicated to providing outstanding in vitro pharmacology services. Based on our excellent experts and advanced instruments, we develop multiple methods to induce hypoxia and for further analysis of drug efficacy.
What Is Hypoxia?
Hypoxia is defined as the deprived or reduction of oxygen in organs, tissues, or cells. It occurs during countless acute and chronic disease states, such as reduced blood flow during stroke or heart attack, and the oxygen-restricted microenvironment within cancers. Hypoxia is also associated with a large number of chronic lung diseases which are major causes of mortality in western populations. The reasons that cause the decreasing of oxygen include defective blood vessel, anemia, and insufficient blood vessel network, or an increased consumption of oxygen relative to the supply. Hypoxia can be physiologic or pathologic, such as rheumatoid arthritis, solid tumor, and atherosclerosis.
Figure 1. Symptoms and signs of hypoxia
In Vitro Hypoxia Studies at Creative Animodel
In vivo hypoxia models test methods are time-consuming, animal intensive and expensive. Over and above, animal models may not be readily adapted to detailed investigations at a molecular and cellular level. Creative Animodel has established various in vitro hypoxia methods to study the effect of different oxygen concentrations and investigate in vitro pharmacology mechanism.
• Hypoxia chambers
Hypoxic chambers have the advantage of not using drugs that can alternate cell behavior independently of the oxygen tension. To create hypoxia, operators will attach tubing to a "hypoxia tank" containing a 1% O2 gas mixture. We have various hypoxic chambers to be chosen according to your projects, space, and other specific conditions.
• Cobalt Chloride methods
The Cobalt Chloride method has the advantages of inexpensive and fast. This method mimics hypoxia by inducing HIF-1/3α but can also regulate other genes. We make sure this method is adapted to your project by checking what other effects it could have on the function and phenotype of your particular cells independently of the "mimic-hypoxic" effect.
• In vitro system
We have made an in vitro system which can mimic the lung environment and investigate the responses of alveolar epithelial cells or microvascular endothelial cells exposed to hypoxia, as these cells are a dominant contributors to chronic pulmonary diseases.
Our in vitro pharmacology evaluation includes, but is not limited to:
• HIF-1 α detection by western blot
• Hypoxia Responsive Element (HRE)
• Positron emission tomography scans
Creative Animodel spends years of hard-working in the field of in vitro pharmacology and devotes to being the best partner for you to conduct hypoxia studies. Our experts are glad to meet your demands from the early-stage of drug development to the final drug discovery phase. If you have any additional requirement or question, please do not hesitate to contact us. We look forward to working with you in the near future.
1. Wu, D.; Yotnda, P. Induction and Testing of Hypoxia in Cell Culture. J. Vis. Exp. 2011, 54(e2899).