Pulmonary Fibrosis Models
Creative Animodel is one of the leading-edge experts among the frontier of pre-clinical model building service. We provide mouse and rat models of bleomycine-induced pulmonary fibrosis for clients all over the world. Based on the experienced scientists and unparalleled technique platforms, Creative Animodel guarantees the customers the most reliable and efficient services to achieve their goals.
Overview of Pulmonary Fibrosis
Pulmonary fibrosis is a chronic and irreversible respiratory disease where the scars are formed in the lung tissue. Along with the process of fibrosis, the tissue around and between the air sacs becomes thickened and stiff, leading to the reduction of oxygen supply in the blood, which further results in progressive shortness of breath. In addition, patients can also suffer from chronic dry cough, fatigue and weakness, discomfort in the chest, aching muscles and joints and unexplained weight loss.
Figure 1. Diagram of pulmonary fibrosis
Pulmonary fibrosis can result from multiple factors including long-term exposure to certain toxins, certain medical conditions, radiation therapy and some medications. However, in most cases of pulmonary fibrosis, the cause is never found, which is called idiopathic pulmonary fibrosis (IPF). Therapies including Pirfenidone and Nintedanib have been approved for IPF treatment, however, the incidence of IPF still keeps increasing. The goals of pulmonary fibrosis intervention for pharmacological researchers are to reduce the symptoms, attenuate or stop disease progression, prevent acute exacerbations, and prolong survival.
Figure 2. Risk factors of pulmonary fibrosis
Bleomycin-induced Pulmonary Fibrosis Models
Bleomycin-induced pulmonary fibrosis, most prevalent in rodent models, is a useful pre-clinical model of human lung fibrosis. Bleomycin is a chemotherapeutic antibiotic, whose application in animal models bases on the fact that fibrosis is one of the major side effects of bleomycin on cancer treatment. Bleomycin leads to inflammation and fibrotic reactions within a short period of time, making it a practical tool for pathobiological studies and novel compounds evaluation in pre-clinical researches. Creative Animodel has developed bleomycin-induced pulmonary fibrosis on mice and rats. The test compound can be delivered by multiple routes including oral, inhalation, intravenous or subcutaneous administration.
Model Characteristics and Pharmacology Evaluation
• In-life parameters of clinical symptoms: respiratory measurement, body weight, daily activity levels, survival…
• Histopathology: fibrotic markers and Ashcroft score
• Collagen quantification: Sircol collagen assay and hydroxyproline assay
• Bronchoalveolar fluid (BALF) analysis: cytological analysis and cytokine ELISA
Creative Animodel offers professional models of pulmonary fibrosis for therapeutic as well as prophylactic studies. We are the best in scientific knowledge, expertise, data quality, timelines, flexibility and personal contacts. We are looking forward to cooperating with you.
1. Moeller A, et al., The bleomycin animal model: a useful tool to investigate treatment options for idiopathic pulmonary fibrosis?. Int J Biochem Cell Biol., 2008, 40 (3): 362-82.